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Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy

Received: 22 September 2016     Accepted: 12 October 2016     Published: 3 November 2016
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Abstract

First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered.

Published in International Journal of Pharmacy and Chemistry (Volume 2, Issue 2)
DOI 10.11648/j.ijpc.20160202.15
Page(s) 31-38
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Anthraquinones, Alkaloids, Fluorescent Drugs, Laser-Scanning Confocal Microscopy, Luminescence Microscopy, Secretory Cells, Sesquiterpene Lactones, Terpenes

References
[1] Roshchina, V. V. Autofluorescence of Plant Secreting Cells as a Biosensor and Bioindicator Reaction. Journal of Fluorescence, 13, p.403 (2003).
[2] Roshchina, V. V. Fluorescing World of Plant Secreting Cells. Science Publishers, Enfield, Plymouth: p. 338 (2008).
[3] Roshchina, V. V. Vital Autofluorescence: Application to the Study of Plant Living Cells. International Journal of Spectroscopy. 2012 ID 124672. p. 1, doi: 10.1155/2012/124672 (2012)
[4] Microscopic Characterization of Botanical Medicines. Eds. Upton R., Graff, A.F., Jolliffe G., Länger, R., Williamson E.M. Boca Raton: CRC Press. 2011. p 800 (2011).
[5] Roshchina, V V., Yashin, V. A., Kuchin, A. V. Fluorescent Analysis for Bioindication of Ozone on Unicellular Models. Jounal of Fluorescence. 25. p. 595, DOI 10.1007/s10895-015-1540-2 (2015).
[6] Karnaukhov, V. N., Yashin, V. A., Kazantsev, A., Karnaukhova, N. A, Kulakov, V. I. Double-wave Microfluorimeter-photometer Based on Standard Attachment. Tsitologia (Cytology, USSR) 29, p. 113 (1987).
[7] Roshchina, V. V. Autofluorescence of Plant Secreting Cells as a Biosensor and Bioindicator Reaction. Journal of Fluorescence, 13, p.403 (2003).
[8] Roshchina, V. V. Fluorescing World of Plant Secreting Cells. Science Publishers, Enfield, Plymouth: p. 338 (2008).
[9] Roshchina, V. V. Vital Autofluorescence: Application to the Study of Plant Living Cells. International Journal of Spectroscopy. 2012 ID 124672. p. 1, doi: 10.1155/2012/124672 (2012)
[10] Microscopic Characterization of Botanical Medicines. Eds. Upton R., Graff, A. F., Jolliffe G., Länger, R., Williamson E. M. Boca Raton: CRC Press. 2011. p 800 (2011).
[11] Roshchina, V V., Melnikova, E. V. Microspectrofluorimetry of Intact Secreting Cells, with Applications to the Study of Allelopathy. In: Inderjit, Dakshini KMM, Foy CL (eds) Principles and Practices in Plant Ecology: Allelochemical interactions. CRC Press, Boca Raton, p 99 (1999)
[12] Póczi, D, Böddi, B. Studies on Laticifers and Milk of Greater Celandine (Chelidonium majus L.) with Fuorescence Imaging and Fluorescence Spectroscopic Methods. Acta Pharm Hung. 80, p.95 (2010).
[13] Kurkin, V. A., Shmygareva, A. A., Ryazanova, T. K., San’kov, A. N. Quantitative Determination of Total Anthracene Derivatives in Frangula Syrup Preparation. Pharmaceutical Chemistry Journal 48, p.467-(2014).
[14] Wagner, H., Bladt, S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. Springer, Berlin, Heidelberg. p.443 (1996).
[15] Francis, W. R, Powers, M. L., Haddock, S. H. Characterization of an Anthraquinone Fluorescence from the Bioluminescent, Pelagic Polychaete Tomopteris. Luminescence. 29, p. 1135 (2014).
[16] Roshchina, V. V, Melnikova, E. V, Spiridonov, N. A., Kovaleva, L. V. Azulenes, the Blue Pigments of Pollen. Doklady Biological Sciences 340, p. 93 (1995)
[17] Roshchina, V. V. Mechanisms of Cell-cell Communication. In: Narwal SS (ed) Allelopathy Update. Vol. 2, Science Publishers, Enfield, New Hampshire, p 3 (1999).
[18] Zia, M., Chaudhary, M. F. Effect of Amino Acids and Plant Growth Regulators on Artemisinin Production in the Callus of Artemisia absinthium. Pakistan J Bot 39, p.799 (2007).
[19] Singh, M. Kr., Saku, P., Nagori, K., Dewangan, D. et al. Organoleptic Properties in-vitro and in-vivo Pharmacological Activities of Calendula officinalis Linn. An over eview. J Chem Pharm Res 3, p. 655 (2011).
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Cite This Article
  • APA Style

    Victoria V. Roshchina, Andrei V. Kuchin, Valerii A. Yashin. (2016). Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy. International Journal of Pharmacy and Chemistry, 2(2), 31-38. https://doi.org/10.11648/j.ijpc.20160202.15

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    ACS Style

    Victoria V. Roshchina; Andrei V. Kuchin; Valerii A. Yashin. Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy. Int. J. Pharm. Chem. 2016, 2(2), 31-38. doi: 10.11648/j.ijpc.20160202.15

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    AMA Style

    Victoria V. Roshchina, Andrei V. Kuchin, Valerii A. Yashin. Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy. Int J Pharm Chem. 2016;2(2):31-38. doi: 10.11648/j.ijpc.20160202.15

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  • @article{10.11648/j.ijpc.20160202.15,
      author = {Victoria V. Roshchina and Andrei V. Kuchin and Valerii A. Yashin},
      title = {Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {2},
      number = {2},
      pages = {31-38},
      doi = {10.11648/j.ijpc.20160202.15},
      url = {https://doi.org/10.11648/j.ijpc.20160202.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20160202.15},
      abstract = {First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Autofluorescence of Plant Secretory Cells as Possible Tool for Pharmacy
    AU  - Victoria V. Roshchina
    AU  - Andrei V. Kuchin
    AU  - Valerii A. Yashin
    Y1  - 2016/11/03
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijpc.20160202.15
    DO  - 10.11648/j.ijpc.20160202.15
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 31
    EP  - 38
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20160202.15
    AB  - First experiments for identification of intact plant secretory cells filled with products of secondary metabolism known as drugs based on their fluorescence characteristics has been analyzed on some medicinal plants. Autofluorescence of compounds containing in intact and dried cells induced, mainly, by ultra-violet or violet light was well seen in visible region under various types of luminescent microscopes, including laser-scanning confocal microscope. In the fluorescence spectra of the objects one could see characteristic maxima that may be used in practice of pharmacy. The autofluorescence application gives a possibility to study occurrence and location of natural drugs within tissues and cells. Examples of similar analysis have been considered by use alkaloid-, anthraquinone- and terpenoid-containing medicinal plant species. Especially informative were the fluorescence spectra of pharmaceutically-valuable Chelidonium majus and Frangula alnus where the emission of alkaloids and anthraquinones, relatively, as drugs prevailed in the natural samples. In first species secretory cells of laticifers contained alkaloids chelerythine and sanguinarine. In more complex, multicomponent samples such as terpenoid-enriched species Achillea millefolium, Artemisia absinthium and Calendula officinalis the interference terpenoids and phenols (brightly fluoresced in glands and secretory hairs) in blue or blue-green emission were observed. Perspectives and difficulties of pharmaceutical analysis with using of autofluorescence were also considered.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Lab. of Micro Spectral Analysis of Cells, Russian Academy of Sciences Institute of Cell Biophysics, Pushchino, Moscow Region, Russia

  • Lab. of Micro Spectral Analysis of Cells, Russian Academy of Sciences Institute of Cell Biophysics, Pushchino, Moscow Region, Russia

  • Lab. of Micro Spectral Analysis of Cells, Russian Academy of Sciences Institute of Cell Biophysics, Pushchino, Moscow Region, Russia

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