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Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity

Received: 11 October 2019     Accepted: 29 October 2019     Published: 13 November 2019
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Abstract

Medicines on the basis of herbs are increasingly used for treating of many diseases, providing the following actions antimicrobial, antiviral, anticancer, anti-enzymatic and immunomodulatory. Ellagitannins are a group of polyphenolic compounds of plant origin, which exhibiting a strong antibacterial effect in virtue of their ability to form a bond with the microbial membrane. Raspberry contains a large amount of ellagitannins, so it is advisable to study their composition and content in raspberry juice, obtained in Ukraine and determine its antimicrobial activity. As raw material Ukrainian variety ‘Phenomenon’ was taken for the research. It was bred in 1991 in Kharkiv region by crossing the varieties ‘Stolichnaya’ and ‘Odarka’. Analysis of raspberry juice ellagitannins was performed by HPLC method. 4 ellagic acid derivatives were identified and their contents were established. Unbound ellagic acid (55% of all) and sanguiin H-6 (21% of all) had the highest content among them Antimicrobial activity of raspberry juice was studied by agar diffusion method. The juice showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The antimicrobial activity of the juice was higher than the comparison drug " Chlorophyllipt " for more than twice in relation to all strains. The greatest effect was manifested relative to Staphylococcus aureus, which can be the cause of purulent-inflammatory processes in almost all human organs with severe consequences. This is especially true in connection with the increasing resistance of strains not only to traditional antibiotics, but also to new groups.

Published in International Journal of Pharmacy and Chemistry (Volume 5, Issue 6)

This article belongs to the Special Issue Drug Research: Current Insights for Search, Development and Analysis

DOI 10.11648/j.ijpc.20190506.11
Page(s) 68-71
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Raspberry, Juice, Ellagitannins, Antimicrobial Activity

References
[1] Okuda T., Yoshida T., Hatano T. (1995). Progress in the chemistry of organic natural products. Springer: New York, NY, USA: 420 p.)
[2] Haslam E. (1989). Plant Polyphenols. Cambridge University Press: Cambridge-UK: 273 p.
[3] Okuda T., Yoshida T., Ashida M. (1981). Casuarictin and casuarinin, two new ellagitannins from Casuarina stricta. Heterocycles № 16: 1681–1685.
[4] Kolodziej H., Kayser O., Kiderlen, A. F., Ito H., Hatano T., Yoshida T., Foo L. Y. (2001). Antileishmanial activity of hydrolyzable tannins and their modulatory effects on nitric oxide and tumor necrosis factor-α release in macrophages in vitro. Planta Med. № 67: 825–832.
[5] Cheng H., Lin C. C., Lin T. C. (2002) Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antiviral Res. № 55: 447–455.
[6] Po-Lin K., Ya-Ling H., Ta-Chen L., Liang-Tzung L., Jiunn Kae C., Chun-Ching L. (2005). Casuarinin from the bark of Terminalia arjuna induces apoptosis and cell cycle arrest in human breast adenocarcinoma MCF-7 cells. Planta Medica № 71: 237–243.
[7] Reddy D. B., Reddanna P. (2009). Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-kappaB and MAPK activation in RAW 264.7 macrophages. Biochem. Biophys. Res. Commun № 381: 112–117.
[8] Cushnie T. P. T., Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents № 26 (5): 343–356.
[9] Chirumbolo S., Bjørklund G., Lysiuk R., Vella A., Lenchyk L., Upyr T. (2018). Targeting Cancer with Phytochemicals via Their Fine Tuning of the Cell Survival Signaling Pathways. International Journal of Molecular Sciences № 19 (11): 3568.
[10] Pharmaceutical encyclopedia. 2nd ed.: Chairman Ed. Council Chernykh V. P. Kiev: “MORION”; 2016: 1952.
[11] Zhang X., Sandhu A., Edirisinghe I., Burton-Freeman B. (2018). An exploratory study of red raspberry (Rubus idaeus L.) (poly) phenols/metabolites in human biological samples. Food & Function № 9 (2): 806–818.
[12] Kula M., Głód D., Krauze-Baranowska M. (2016). Two-dimensional liquid chromatography (LC) of phenolic compounds from the shoots of Rubus idaeus “Glen Ample” cultivar variety. Journal of Pharmaceutical and Biomedical Analysis № 121: 99–106.
[13] Zhbanova E. V. (2018). Fruit of raspberry Rubus idaeus L. as a source of functional ingredients (review) Food Processing: Techniques and Technology. Vol. 48. № 1: 5-14.
[14] Sójka M., Macierzyński J., Zaweracz W., Buczek M. (2016). Transfer and Mass Balance of Ellagitannins, Anthocyanins, Flavan-3-ols, and Flavonols during the Processing of Red Raspberries (Rubus idaeus L.) to Juice. Journal of Agricultural and Food Chemistry №64 (27): 5549–5563.
[15] Yeromina H. O., Upyr T. V., Osolodchenko T. P., Ieromina Z. G., Demchenko N. R., Perekhoda L. O. (2019). The search for substances with the antimicrobial activity among morpholine-containing 2-R-phenyliminothiazole derivatives. Journal of organic and pharmaceutical chemistry Vol. 17, Iss. 1 (65): 58–62.
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  • APA Style

    Ivan Polischuk, Mykola Komisarenko, Taras Upyr, Alla Kovaleva, Andriy Komisarenko. (2019). Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity. International Journal of Pharmacy and Chemistry, 5(6), 68-71. https://doi.org/10.11648/j.ijpc.20190506.11

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    ACS Style

    Ivan Polischuk; Mykola Komisarenko; Taras Upyr; Alla Kovaleva; Andriy Komisarenko. Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity. Int. J. Pharm. Chem. 2019, 5(6), 68-71. doi: 10.11648/j.ijpc.20190506.11

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    AMA Style

    Ivan Polischuk, Mykola Komisarenko, Taras Upyr, Alla Kovaleva, Andriy Komisarenko. Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity. Int J Pharm Chem. 2019;5(6):68-71. doi: 10.11648/j.ijpc.20190506.11

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  • @article{10.11648/j.ijpc.20190506.11,
      author = {Ivan Polischuk and Mykola Komisarenko and Taras Upyr and Alla Kovaleva and Andriy Komisarenko},
      title = {Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {5},
      number = {6},
      pages = {68-71},
      doi = {10.11648/j.ijpc.20190506.11},
      url = {https://doi.org/10.11648/j.ijpc.20190506.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20190506.11},
      abstract = {Medicines on the basis of herbs are increasingly used for treating of many diseases, providing the following actions antimicrobial, antiviral, anticancer, anti-enzymatic and immunomodulatory. Ellagitannins are a group of polyphenolic compounds of plant origin, which exhibiting a strong antibacterial effect in virtue of their ability to form a bond with the microbial membrane. Raspberry contains a large amount of ellagitannins, so it is advisable to study their composition and content in raspberry juice, obtained in Ukraine and determine its antimicrobial activity. As raw material Ukrainian variety ‘Phenomenon’ was taken for the research. It was bred in 1991 in Kharkiv region by crossing the varieties ‘Stolichnaya’ and ‘Odarka’. Analysis of raspberry juice ellagitannins was performed by HPLC method. 4 ellagic acid derivatives were identified and their contents were established. Unbound ellagic acid (55% of all) and sanguiin H-6 (21% of all) had the highest content among them Antimicrobial activity of raspberry juice was studied by agar diffusion method. The juice showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The antimicrobial activity of the juice was higher than the comparison drug " Chlorophyllipt " for more than twice in relation to all strains. The greatest effect was manifested relative to Staphylococcus aureus, which can be the cause of purulent-inflammatory processes in almost all human organs with severe consequences. This is especially true in connection with the increasing resistance of strains not only to traditional antibiotics, but also to new groups.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity
    AU  - Ivan Polischuk
    AU  - Mykola Komisarenko
    AU  - Taras Upyr
    AU  - Alla Kovaleva
    AU  - Andriy Komisarenko
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    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 68
    EP  - 71
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20190506.11
    AB  - Medicines on the basis of herbs are increasingly used for treating of many diseases, providing the following actions antimicrobial, antiviral, anticancer, anti-enzymatic and immunomodulatory. Ellagitannins are a group of polyphenolic compounds of plant origin, which exhibiting a strong antibacterial effect in virtue of their ability to form a bond with the microbial membrane. Raspberry contains a large amount of ellagitannins, so it is advisable to study their composition and content in raspberry juice, obtained in Ukraine and determine its antimicrobial activity. As raw material Ukrainian variety ‘Phenomenon’ was taken for the research. It was bred in 1991 in Kharkiv region by crossing the varieties ‘Stolichnaya’ and ‘Odarka’. Analysis of raspberry juice ellagitannins was performed by HPLC method. 4 ellagic acid derivatives were identified and their contents were established. Unbound ellagic acid (55% of all) and sanguiin H-6 (21% of all) had the highest content among them Antimicrobial activity of raspberry juice was studied by agar diffusion method. The juice showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The antimicrobial activity of the juice was higher than the comparison drug " Chlorophyllipt " for more than twice in relation to all strains. The greatest effect was manifested relative to Staphylococcus aureus, which can be the cause of purulent-inflammatory processes in almost all human organs with severe consequences. This is especially true in connection with the increasing resistance of strains not only to traditional antibiotics, but also to new groups.
    VL  - 5
    IS  - 6
    ER  - 

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Author Information
  • Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Pharmacognosy, National University of Pharmacy, Kharkiv, Ukraine

  • Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, Kharkiv, Ukraine

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