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Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya

Received: 17 November 2022     Accepted: 4 February 2023     Published: 27 February 2023
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Abstract

Lamiaceae family comprise a wide range of medicinal and aromatic plant species with strong antioxidant properties and multiple pharmaceutical applications in folk medicine. Mentha piperita L., Ocimum basilicum, Origanum vulgare L, Rosmarinus officinalis L, Salvia officinalis L. and Thymus capitatus, are edible plants in Libya, belonging to this family. The current work aims to evaluate gallic acid and rutin contents of the methanolic extract of the mentioned plants using RP- High performance liquid chromatography (HPLC) and assess their antioxidant properties by applying the DPPH radical scavenging assay. The content of gallic acid were found to be considerable in all plants studied, most remarkably in Rosmarinus officinalis L. and Thymus capitatus with area under the peak (56.14% and 54.62% respectively), while lower contents were detected in Ocimum basilicum (34.44%), Origanum vulgare L. (28.98%), Mentha piperita L.(26.09%), and Salvia officinalis L. (24.34%). On the other hand, it was noted that rutin is found in less quantities compared to previous results. The extracts of Organium vulgare and Thymus capitatus had a significant antioxidant potential compared to standard vitamin C with 0.34 ± 0.03 and 0.46 ± 0.02 mg/mL respectively, while a lower scavenging capacity were recoded for Mentha piperita L. (0.59 mg/mL ± 0.01), Salvia officinalis L. (0.60 mg/mL ± 0.01), Rosmarinus officinalis L (0.65 mg/mL ± 0.07), and Ocimum basilicum (0.99 mg/mL ± 0.02). In conclusion, the reported results point out the significance of this family as a source of antioxidant agents with high promising capability to affect the redox state.

Published in International Journal of Pharmacy and Chemistry (Volume 9, Issue 1)
DOI 10.11648/j.ijpc.20230901.11
Page(s) 1-8
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Libya, Gallic Acid, Rutin, DPPH, HPLC

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    Abdullah Alamami, Samar Ben Zaed, Esra El Naili, Areej Daboub, Naema Elremali, et al. (2023). Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya. International Journal of Pharmacy and Chemistry, 9(1), 1-8. https://doi.org/10.11648/j.ijpc.20230901.11

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    Abdullah Alamami; Samar Ben Zaed; Esra El Naili; Areej Daboub; Naema Elremali, et al. Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya. Int. J. Pharm. Chem. 2023, 9(1), 1-8. doi: 10.11648/j.ijpc.20230901.11

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    AMA Style

    Abdullah Alamami, Samar Ben Zaed, Esra El Naili, Areej Daboub, Naema Elremali, et al. Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya. Int J Pharm Chem. 2023;9(1):1-8. doi: 10.11648/j.ijpc.20230901.11

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  • @article{10.11648/j.ijpc.20230901.11,
      author = {Abdullah Alamami and Samar Ben Zaed and Esra El Naili and Areej Daboub and Naema Elremali and Fatma Elshibani and Salim Skaik},
      title = {Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya},
      journal = {International Journal of Pharmacy and Chemistry},
      volume = {9},
      number = {1},
      pages = {1-8},
      doi = {10.11648/j.ijpc.20230901.11},
      url = {https://doi.org/10.11648/j.ijpc.20230901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20230901.11},
      abstract = {Lamiaceae family comprise a wide range of medicinal and aromatic plant species with strong antioxidant properties and multiple pharmaceutical applications in folk medicine. Mentha piperita L., Ocimum basilicum, Origanum vulgare L, Rosmarinus officinalis L, Salvia officinalis L. and Thymus capitatus, are edible plants in Libya, belonging to this family. The current work aims to evaluate gallic acid and rutin contents of the methanolic extract of the mentioned plants using RP- High performance liquid chromatography (HPLC) and assess their antioxidant properties by applying the DPPH radical scavenging assay. The content of gallic acid were found to be considerable in all plants studied, most remarkably in Rosmarinus officinalis L. and Thymus capitatus with area under the peak (56.14% and 54.62% respectively), while lower contents were detected in Ocimum basilicum (34.44%), Origanum vulgare L. (28.98%), Mentha piperita L.(26.09%), and Salvia officinalis L. (24.34%). On the other hand, it was noted that rutin is found in less quantities compared to previous results. The extracts of Organium vulgare and Thymus capitatus had a significant antioxidant potential compared to standard vitamin C with 0.34 ± 0.03 and 0.46 ± 0.02 mg/mL respectively, while a lower scavenging capacity were recoded for Mentha piperita L. (0.59 mg/mL ± 0.01), Salvia officinalis L. (0.60 mg/mL ± 0.01), Rosmarinus officinalis L (0.65 mg/mL ± 0.07), and Ocimum basilicum (0.99 mg/mL ± 0.02). In conclusion, the reported results point out the significance of this family as a source of antioxidant agents with high promising capability to affect the redox state.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya
    AU  - Abdullah Alamami
    AU  - Samar Ben Zaed
    AU  - Esra El Naili
    AU  - Areej Daboub
    AU  - Naema Elremali
    AU  - Fatma Elshibani
    AU  - Salim Skaik
    Y1  - 2023/02/27
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ijpc.20230901.11
    DO  - 10.11648/j.ijpc.20230901.11
    T2  - International Journal of Pharmacy and Chemistry
    JF  - International Journal of Pharmacy and Chemistry
    JO  - International Journal of Pharmacy and Chemistry
    SP  - 1
    EP  - 8
    PB  - Science Publishing Group
    SN  - 2575-5749
    UR  - https://doi.org/10.11648/j.ijpc.20230901.11
    AB  - Lamiaceae family comprise a wide range of medicinal and aromatic plant species with strong antioxidant properties and multiple pharmaceutical applications in folk medicine. Mentha piperita L., Ocimum basilicum, Origanum vulgare L, Rosmarinus officinalis L, Salvia officinalis L. and Thymus capitatus, are edible plants in Libya, belonging to this family. The current work aims to evaluate gallic acid and rutin contents of the methanolic extract of the mentioned plants using RP- High performance liquid chromatography (HPLC) and assess their antioxidant properties by applying the DPPH radical scavenging assay. The content of gallic acid were found to be considerable in all plants studied, most remarkably in Rosmarinus officinalis L. and Thymus capitatus with area under the peak (56.14% and 54.62% respectively), while lower contents were detected in Ocimum basilicum (34.44%), Origanum vulgare L. (28.98%), Mentha piperita L.(26.09%), and Salvia officinalis L. (24.34%). On the other hand, it was noted that rutin is found in less quantities compared to previous results. The extracts of Organium vulgare and Thymus capitatus had a significant antioxidant potential compared to standard vitamin C with 0.34 ± 0.03 and 0.46 ± 0.02 mg/mL respectively, while a lower scavenging capacity were recoded for Mentha piperita L. (0.59 mg/mL ± 0.01), Salvia officinalis L. (0.60 mg/mL ± 0.01), Rosmarinus officinalis L (0.65 mg/mL ± 0.07), and Ocimum basilicum (0.99 mg/mL ± 0.02). In conclusion, the reported results point out the significance of this family as a source of antioxidant agents with high promising capability to affect the redox state.
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Department of Basic Medical Science, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya

  • Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, University of Zawia, Tripoli, Libya

  • Department of Chemistry, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya

  • Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, University of Zawia, Tripoli, Libya

  • Department of Chemistry, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya

  • Department of Pharmacognosy, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya

  • Department of Pharmacognosy, Faculty of Pharmacy, Assalam International University, Benghazi, Libya

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